Shasta - Replacing an Amidite Line

Tools & Materials

The following tools and materials are required to complete this procedure:

• Screwdriver set (Phillips)
• ANSI Allen Keys
• Replacement liquid line tubing
• P-287 Idex Super flangeless fittings
• P-259 steel compression rings and ferrules
• Pliers
• Labeling materials (if transferring labels)

• Wear appropriate personal protective equipment (PPE) such as gloves and safety glasses.
• Follow manufacturer guidelines for handling hazardous materials.

Procedure

1. Remove all liquid from the liquid line
   1. Place an empty bottle on the troublesome position.
   2. Access the priming screen in the software and prime all liquid out of the line
      
      
2. Depressurize the instrument
   1. Turn the ball valve for Amidite pressure to the off position
   2. Depressurize the instrument by removing the bottle from the troublesome location
3. Remove the Front Rack Sheet Metal
   1. Remove screws from the top of the front bottle rack sheet metal.
      
      
      
      
   2. Remove screws along the underside of the front bottle rack.
      
      
   3. Attempt to gently pull the sheet metal off.
      

      Note: If stuck (especially on the right side), proceed to Step 4 to access hidden screws. Otherwise proceed to step 5

       

4. Access Hidden Screws (if needed)
   1. Remove the side panel covering the electrical enclosure.
      
      
   2. Identify and remove up to 3 screws securing the interior sheet metal to the chamber.
      
   3. Remove screws along the top and underside of the back panel and side sections.
      
   4. Gently remove the side/back sheet metal panel to expose the remaining hidden screws.
      
      
      
      
5. Remove Bottle Rack
   1. Remove the front cover completely.
      
   2. Remove bottles to access mounting screws.
      
   3. Unscrew and gently pull the bottle rack away, setting it on a table for support.
      
      
6. Identify and Remove Liquid Line
   1. Locate the liquid line for the position you are troubleshooting
   2. 
   3. Pull the liquid line out from the top of the receptacle.
7. Trace and Remove Liquid Line
   1. Open side panel covering fluid pathways.
   2. Trace liquid line to Y connector.
   3. Remove all affected lines from Y fitting, dispense valves, and tips.
   4. Cut and prepare new tubing to match original lengths.
   5. Transfer any labels, if possible.
8. Prepare New Tubing Ends
   1. Slide nut, compression ring, and ferrule onto tubing (in that order).
      
   2. Flattened side of ring should face nut; narrow side of ferrule toward ring.
   3. Use Y fitting or Peek union to seal ferrule/compression ring onto tubing.
      
9. Reinsert Tubing into Bottle Cap
   1. Stretch the tubing end by looping around finger and pulling with pliers.
      
      
   2. Trim the flattened tip and feed stretched section through cap hole.
   3. Pull tubing through to bottle side using pliers.
      
      
   4. Ensure enough tubing reaches bottle bottom, trim excess length.
10. Final Connections
    1. Connect all new lines to appropriate dispense tips, valves and Y fitting
11. Reassembly
    1. Reassemble sheet metal panels in reverse order of disassembly
12. Validation & Testing
    
    1. Turn on the equipment if it is not on already
    2. Place a bottle with liquid on the position corresponding to the line that was just replaced if not done already.
    3. Pressurize the amidite bottles again by turning the ball valve to the on position.
    4. Verify there are no leaks at any of the connection points for the line that was just replaced. (It might be helpful to leave side panels off)
    5. Open the Software and access the Priming Menu
       
    6. Select the valve that corresponds to the amidite line that was just replaced
    7. Prime the valve and verify that the liquid is injecting into the waste tray
    8. Again verify no leaks at any connection points.