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K&A - Power, Air, Space and Environmental Requirements

This article outlines the site and laboratory requirements for installing and operating K&A / H-Series oligonucleotide synthesizers. Proper preparation ensures stable performance, reliable reagent flow, and a smooth installation.

1. Electrical Power Requirements

Standard Power

Voltage:
  • 115 VAC, 50/60 Hz (North America)
  • 220–230 VAC, 50/60 Hz (International)
  • Outlet: Standard grounded outlet
  • Dedicated Circuit: Strongly recommended
Typical Load
  • Low overall power consumption
  • Peak draw typically <5 A at 115 V
  • No three-phase power required
Best Practices
  • Use a surge protector
  • Avoid shared circuits with ovens, centrifuges, or freezers


2. Compressed Air Requirements

K&A / H-Series systems rely on compressed air for valve actuation. A compressor is supplied with the instrument. A High pressure source can be substituted for the compressor as long as it can supply a consistent pressure at a range of 60-100psi

 

Compressed Air or High Pressure source
  • Operating Pressure:
    • 0.4–0.5 MPa (60–75 psi)
    • Air Quality:
  • Clean, dry, oil-free air
  • Recommended filtration: ≤5 µm
  • Flow Demand:
    • Low continuous flow
    • Suitable for small laboratory compressors
Compressor Guidance
  • Quiet, lab-rated compressors are recommended
  • Regular draining and maintenance are required to prevent moisture issues

Gas Requirements

Argon or Nitrogen
  • Grade 5 purity or higher
  • Used for bottle  pressurization
  • Typical Input Pressure:25-30PSI
  • Standard lab argon cylinder with dual-stage regulator. Drop down 1-stage regulator can be used if using in-house pressure system is used



3. Space & Benchtop Requirements

Bench Space
  • Minimum Width: ~36 in (90 cm)

  • Recommended Width: 48–60 in (120–150 cm)

  • Depth: ≥30 in (75 cm)

  • Vertical Clearance: ≥30 in (75 cm)

Bench Requirements
  • Rigid, level, vibration-free surface
  • Chemical-resistant benchtop preferred
Clearance
  • Front or side access for:
    • Column loading
    • Bottle changes
    • Routine service


4. Environmental Conditions

  • Temperature: 18–25 °C (64–77 °F)
  • Humidity: <80% RH, non-condensing
  • Ventilation:
  • Standard lab ventilation required
  • Local exhaust or hood recommended for volatile reagents
  • Vibration: Avoid proximity to shakers or centrifuges