--- title: K&A - Maintenance Schedule description: K&A - Maintenance Schedule --- [Skip to content](https://support.sierrabio.com/ka-maintenance-schedule#main-content) English Show submenu for translations ![](https://support.sierrabio.com/hs-fs/hubfs/JPG%20file%20-02.jpg?width=2000&height=1125&name=JPG%20file%20-02.jpg) Open main navigation Close main navigation - English Show submenu for translations - [Contact us](mailto:support@sierrabio.com) [Contact us](mailto:support@sierrabio.com) How can we help you? - There are no suggestions because the search field is empty. 1. [Sierra Biosystems Support Center](https://support.sierrabio.com/?hsLang=en) 2. [Troubleshooting & Errors](https://support.sierrabio.com/troubleshooting-errors?hsLang=en) 3. [K&A - Preventative Maintenance & Quick Fixes](https://support.sierrabio.com/troubleshooting-errors?hsLang=en#ka-preventative-maintenance-quick-fixes) August 13, 2025 # K&A - Maintenance Schedule ## This guide outlines recommended maintenance tasks and their ideal frequency. ### **🗓️ Weekly Maintenance** ### Check Reagent & Amidite Levels - Verify that all bottles are filled to appropriate levels - Refill or replace any reagents nearing depletion ### Visual Inspection - Inspect the instrument for leaks, salt buildup, or chemical residue - Confirm that no hoses or fittings appear loose or damaged ### Run Shut Down Procedure - If the instrument will not be used for some time. perform a complete flush of the fluid lines using the Shutdown Procedure in the Manual Control Screen. ![](https://support.sierrabio.com/hs-fs/hubfs/image-png-Jul-21-2025-12-28-19-5745-AM.png?width=521&height=211&name=image-png-Jul-21-2025-12-28-19-5745-AM.png) --- ### **🗓️ Monthly Maintenance** ### O-Ring and Seal Inspection - Inspect seals on amidite and reagent bottle caps ### Check Pressure Stability - Refer to [THIS VIDEO](https://47834229.hs-sites.com/ka-troubleshooting-gas-leaks?hsLang=en) - Pressurize the system and monitor for pressure drop - A drop of more than 0.5 psi in 1 minute may indicate a leak ### Valve Function Test - Use manual valve control to ensure each valve actuates with a clear click ![](https://support.sierrabio.com/hs-fs/hubfs/image-png-Jul-21-2025-12-26-59-6833-AM.png?width=670&height=242&name=image-png-Jul-21-2025-12-26-59-6833-AM.png) - Check for steady flow and even delivery from each valve ### **Flow Test** - Perform a calibration check using a precision balance and graduated vial. Refer to [THIS PAGE.](https://47834229.hs-sites.com/ka-how-to-perform-a-flow-test?hsLang=en) - Dispense and measure output for each valve - Address any inconsistencies. - If a Valve Replacement is necessary refer to [THIS VIDEO.](https://47834229.hs-sites.com/ka-how-to-replace-a-valve?hsLang=en) Remove water and check oil level of Compressor - Refer to [THIS PAGE](https://47834229.hs-sites.com/ka-monthly-compressor-maintenance-procedure?hsLang=en) - Turn off the compressor - Hold a collection vial underneath the line for the purge valve - Open the Manual Water Remove Switch to remove all water - Close the switch - Turn the compressor back on --- ### 🗓️ 6 Month Maintenance ### **Inspect Manifolds and Internal Connections** - Open the instrument and inspect reagent manifolds and pneumatic fittings - Look for signs of wear, leaks, or corrosion. ### Replace Wear Components - Replace any valves  with inconsistent performance. Refer to [THIS VIDEO.](https://47834229.hs-sites.com/ka-how-to-replace-a-valve?hsLang=en) - Replace Main Delivery Manifold Membrane. Refer to [THIS PAGE.](https://47834229.hs-sites.com/ka-how-to-change-a-membrane?hsLang=en) ### Software Backup - Backup configuration files and job data --- ### 🗓️ Annual Maintenance ### Replace Wear Components - Replace O-rings, seals, and any tubing showing signs of fatigue - Replace any valves or regulators with inconsistent performance. Refer to [THIS VIDEO.](https://47834229.hs-sites.com/ka-how-to-replace-a-valve?hsLang=en) - Replace ALL Membranes (Liquid Delivery Manifold and Waste Manifolds). Refer to [THIS PAGE.](https://47834229.hs-sites.com/ka-how-to-change-a-membrane?hsLang=en) ### Deep System Clean - Flush all fluidic paths thoroughly - Clean interior and exterior surfaces using appropriate solvents --- ### 🧪 Additional Tips - Use only anhydrous acetonitrile or other compatible solvents - Always wear appropriate PPE during maintenance procedures - Log all maintenance actions in the system service log - [Pre-Installation & Installation](https://support.sierrabio.com/pre-installation-installation?hsLang=en#main-content) - [Pre-Installation Requirements](https://support.sierrabio.com/pre-installation-installation?hsLang=en#pre-installation-requirements) - [Site & Lab Preparation](https://support.sierrabio.com/pre-installation-installation?hsLang=en#site-lab-preparation) - [Installation & Initial Setup](https://support.sierrabio.com/pre-installation-installation?hsLang=en#installation-initial-setup) - [Troubleshooting & Errors](https://support.sierrabio.com/troubleshooting-errors?hsLang=en#main-content) - [Software Errors & Communication Issues](https://support.sierrabio.com/troubleshooting-errors?hsLang=en#software-errors-communication-issues) - [Chemistry and Protocol-Related Issues](https://support.sierrabio.com/troubleshooting-errors?hsLang=en#chemistry-and-protocol-related-issues) - [K&A - Preventative Maintenance & Quick Fixes](https://support.sierrabio.com/troubleshooting-errors?hsLang=en#ka-preventative-maintenance-quick-fixes) - [Shasta - Preventative Maintenance & Quick Fixes](https://support.sierrabio.com/troubleshooting-errors?hsLang=en#shasta-preventative-maintenance-quick-fixes) - [Shasta - Hardware Issues](https://support.sierrabio.com/troubleshooting-errors?hsLang=en#shasta-hardware-issues) - [Training Videos and Guides](https://support.sierrabio.com/training-videos-and-guides?hsLang=en#main-content) - [Shasta Software](https://support.sierrabio.com/training-videos-and-guides?hsLang=en#shasta-software) - [K&A Next Gen Software](https://support.sierrabio.com/training-videos-and-guides?hsLang=en#ka-next-gen-software) - [K&A Legacy Software](https://support.sierrabio.com/training-videos-and-guides?hsLang=en#ka-legacy-software) - [K&A - Troubleshooting Videos](https://support.sierrabio.com/training-videos-and-guides?hsLang=en#ka-troubleshooting-videos) - [Shasta - Troubleshooting Videos](https://support.sierrabio.com/training-videos-and-guides?hsLang=en#shasta-troubleshooting-videos) - [Software Updates & Release Notes](https://support.sierrabio.com/software-updates-release-notes?hsLang=en#main-content) - [Release Notes](https://support.sierrabio.com/software-updates-release-notes?hsLang=en#release-notes) - [Downloads](https://support.sierrabio.com/software-updates-release-notes?hsLang=en#downloads) - [Update Instructions](https://support.sierrabio.com/software-updates-release-notes?hsLang=en#update-instructions) - [Product Documentation & Policies](https://support.sierrabio.com/product-documentation-policies?hsLang=en#main-content) - [Technical Documentation](https://support.sierrabio.com/product-documentation-policies?hsLang=en#technical-documentation) - [Specifications](https://support.sierrabio.com/product-documentation-policies?hsLang=en#specifications) - [Warranty & Service](https://support.sierrabio.com/product-documentation-policies?hsLang=en#warranty-service) - [Oligonucleotide Chemistry & Methods](https://support.sierrabio.com/oligonucleotide-chemistry-methods?hsLang=en#main-content) - [Synthesis & Strategy](https://support.sierrabio.com/oligonucleotide-chemistry-methods?hsLang=en#synthesis-strategy) - [Post-Synthesis Processing](https://support.sierrabio.com/oligonucleotide-chemistry-methods?hsLang=en#post-synthesis-processing) - [Quantitation & QC](https://support.sierrabio.com/oligonucleotide-chemistry-methods?hsLang=en#quantitation-qc) - [Special Topics](https://support.sierrabio.com/oligonucleotide-chemistry-methods?hsLang=en#special-topics) - [Support & Contact](https://support.sierrabio.com/support-contact?hsLang=en) - [Uncategorized](https://support.sierrabio.com/uncategorized?hsLang=en) [![](https://support.sierrabio.com/hs-fs/hubfs/JPG%20file%20-02.jpg?width=2000&height=1125&name=JPG%20file%20-02.jpg)](http://sierrabio.com) [mailto:Support@sierrabio.com](mailto:Support@sierrabio.com) Copyright © 2025, Sierra BioSystems