--- title: Troubleshooting & Errors | Sierra Biosystems Support Center description: Look at troubleshooting suggestions to common issues and error codes --- [Skip to content](https://support.sierrabio.com/troubleshooting-errors#main-content) English Show submenu for translations ![](https://support.sierrabio.com/hs-fs/hubfs/JPG%20file%20-02.jpg?width=2000&height=1125&name=JPG%20file%20-02.jpg) Open main navigation Close main navigation - English Show submenu for translations - [Contact us](mailto:support@sierrabio.com) [Contact us](mailto:support@sierrabio.com) How can we help you? - There are no suggestions because the search field is empty. 1. [Sierra Biosystems Support Center](https://support.sierrabio.com/) 2. [Troubleshooting & Errors](https://support.sierrabio.com/troubleshooting-errors) # Troubleshooting & Errors Look at troubleshooting suggestions to common issues and error codes - [Installing Flangeless Ferrule Fittings](https://support.sierrabio.com/installing-flangeless-ferrule-fittings) - [Pressure Imbalance on K&A Synthesizers](https://support.sierrabio.com/pressure-imbalance-on-ka-synthesizers) ## Software Errors & Communication Issues - [Troubleshooting NexusDB Server Issues](https://support.sierrabio.com/troubleshooting-nexusdb-server-issues) - [Shasta Software Errors](https://support.sierrabio.com/shasta-software-errors) - [How to Help Us Identify Software or Computer Issues](https://support.sierrabio.com/how-to-help-us-identify-software-or-computer-issues) - [Troubleshooting Server Connection Errors](https://support.sierrabio.com/troubleshooting-server-connection-errors) - [K&A Software Errors](https://support.sierrabio.com/ka-software-errors) ## Chemistry and Protocol-Related Issues - [Common Mass Impurities Observed in Oligonucleotide Analysis](https://support.sierrabio.com/common-mass-spectrometry-impurities-observed-in-oligonucleotide-analysis) - [Sulfurization Strategies for Phosphorothioate (PS) Oligonucleotide Synthesis](https://support.sierrabio.com/sulfurization-strategies-for-phosphorothioate-oligonucleotide-synthesis) - [Optimizing Protocols in K&A or Shasta Software](https://support.sierrabio.com/optimizing-protocols-in-ka-or-shasta-software) - [Troubleshooting Poor Yields, Impurities and Methods](https://support.sierrabio.com/troubleshooting-poor-yields-impurities-and-methods) ## K&A - Preventative Maintenance & Quick Fixes - [K&A - Understanding the Shut Down Procedure](https://support.sierrabio.com/ka-understanding-the-shut-down-procedure) - [K&A - Understanding Trityl Monitors](https://support.sierrabio.com/ka-understanding-trityl-monitors) - [K&A - Monthly Compressor Maintenance Procedure](https://support.sierrabio.com/ka-monthly-compressor-maintenance-procedure) - [K&A - How to Perform a Flow Test](https://support.sierrabio.com/ka-how-to-perform-a-flow-test) - [K&A - Understanding Priming and Prime Times in K&A Synthesizers](https://support.sierrabio.com/understanding-priming-and-prime-times-in-ka-synthesizers) - [K&A - How To Change a Membrane](https://support.sierrabio.com/ka-how-to-change-a-membrane) - [K&A - Maintenance Schedule](https://support.sierrabio.com/ka-maintenance-schedule) - [K&A - Replacing or Adding a Bottle Cap](https://support.sierrabio.com/ka-replacing-a-bottle-cap) See less ▼ ## Shasta - Preventative Maintenance & Quick Fixes - [Shasta - Monthly Maintenance Procedure](https://support.sierrabio.com/monthly-maintenance-procedure-shasta-dna/rna-synthesizer) - [Shasta - How to Replace a Clogged Line or Valve on a Shasta Synthesizer](https://support.sierrabio.com/how-to-replace-a-clogged-line-or-valve-on-a-shasta-synthesizer) - [How to Clean Vent Holes Using a Guitar String](https://support.sierrabio.com/how-to-clean-vent-holes-using-a-guitar-string) - [How to Minimize Argon Usage on Shasta Systems](https://support.sierrabio.com/how-to-minimize-argon-usage-on-shasta-systems) - [Shasta - Replacing Bottle-to-Valve Amidite Line](https://support.sierrabio.com/shasta-replacing-an-amidite-line) ## Shasta - Hardware Issues - [Synthesis Slowing Down Over Time – Possible Y‑Axis Cable Connection Issue](https://support.sierrabio.com/synthesis-slowing-down-over-time-possible-y-axis-cable-connection-issue) - [Pre-Installation & Installation](https://support.sierrabio.com/pre-installation-installation#main-content) - [Pre-Installation Requirements](https://support.sierrabio.com/pre-installation-installation#pre-installation-requirements) - [Site & Lab Preparation](https://support.sierrabio.com/pre-installation-installation#site-lab-preparation) - [Installation & Initial Setup](https://support.sierrabio.com/pre-installation-installation#installation-initial-setup) - [Troubleshooting & Errors](https://support.sierrabio.com/troubleshooting-errors#main-content) - [Software Errors & Communication Issues](https://support.sierrabio.com/troubleshooting-errors#software-errors-communication-issues) - [Chemistry and Protocol-Related Issues](https://support.sierrabio.com/troubleshooting-errors#chemistry-and-protocol-related-issues) - [K&A - Preventative Maintenance & Quick Fixes](https://support.sierrabio.com/troubleshooting-errors#ka-preventative-maintenance-quick-fixes) - [Shasta - Preventative Maintenance & Quick Fixes](https://support.sierrabio.com/troubleshooting-errors#shasta-preventative-maintenance-quick-fixes) - [Shasta - Hardware Issues](https://support.sierrabio.com/troubleshooting-errors#shasta-hardware-issues) - [Training Videos and Guides](https://support.sierrabio.com/training-videos-and-guides#main-content) - [Shasta Software](https://support.sierrabio.com/training-videos-and-guides#shasta-software) - [K&A Next Gen Software](https://support.sierrabio.com/training-videos-and-guides#ka-next-gen-software) - [K&A Legacy Software](https://support.sierrabio.com/training-videos-and-guides#ka-legacy-software) - [K&A - Troubleshooting Videos](https://support.sierrabio.com/training-videos-and-guides#ka-troubleshooting-videos) - [Shasta - Troubleshooting Videos](https://support.sierrabio.com/training-videos-and-guides#shasta-troubleshooting-videos) - [Software Updates & Release Notes](https://support.sierrabio.com/software-updates-release-notes#main-content) - [Release Notes](https://support.sierrabio.com/software-updates-release-notes#release-notes) - [Downloads](https://support.sierrabio.com/software-updates-release-notes#downloads) - [Update Instructions](https://support.sierrabio.com/software-updates-release-notes#update-instructions) - [Product Documentation & Policies](https://support.sierrabio.com/product-documentation-policies#main-content) - [Technical Documentation](https://support.sierrabio.com/product-documentation-policies#technical-documentation) - [Specifications](https://support.sierrabio.com/product-documentation-policies#specifications) - [Warranty & Service](https://support.sierrabio.com/product-documentation-policies#warranty-service) - [Oligonucleotide Chemistry & Methods](https://support.sierrabio.com/oligonucleotide-chemistry-methods#main-content) - [Synthesis & Strategy](https://support.sierrabio.com/oligonucleotide-chemistry-methods#synthesis-strategy) - [Post-Synthesis Processing](https://support.sierrabio.com/oligonucleotide-chemistry-methods#post-synthesis-processing) - [Quantitation & QC](https://support.sierrabio.com/oligonucleotide-chemistry-methods#quantitation-qc) - [Special Topics](https://support.sierrabio.com/oligonucleotide-chemistry-methods#special-topics) - [Support & Contact](https://support.sierrabio.com/support-contact) - [Uncategorized](https://support.sierrabio.com/uncategorized) [![](https://support.sierrabio.com/hs-fs/hubfs/JPG%20file%20-02.jpg?width=2000&height=1125&name=JPG%20file%20-02.jpg)](http://sierrabio.com) [mailto:Support@sierrabio.com](mailto:Support@sierrabio.com) Copyright © 2025, Sierra BioSystems