Shasta Software - Release Notes - V. 2.5.3

🚀 New in v2.5.3

• Sequence Editor Fix: Resolved issue preventing column numbers >24 from being entered.

• UI Default Behavior: "Add ACN to completed wells" now defaults to OFF.

• Custom Amidite Naming: Allowed “-” in custom amidite names; removed it as a reserved modifier.

• Post-Synthesis Plate Movement: Plate now moves to neutral position (100,100) after synthesis to avoid tip drips.

• New Plate Type: Introduced "2x96v2" plate type with adjusted gap spacing.

• Dual-Plate Handling:

  • 2x96: 3-column gap (2700mm) – unchanged.

  • 2x96v2: 6-column gap (5400mm) – new.

  • 2x384: 11-column gap (4950mm) – increased from 6 columns.

  • Plate offset calibration now uses gap columns, not X-offset.

• Naming Flexibility: Reagents and Protocols now support “-” in names for Mix and Specials.

• Protocol Detail Loading Fix: Resolved issue with details not loading in Run Synthesis screen.

• Security Update: Removed support for NetBIOS computer names due to Microsoft deprecation.

• 64-Well Plate Support:

  • Added 64-well plate option in Configuration and Run Synthesis screens.

  • Implemented unique vertical drain group pattern:
    1, 5, 9, 13, 3, 7, 11, 15, 2, 6, 10, 14, 4, 8, 12, 16

  • Adjusted Manual Control and Map Protocol UIs with A–P drain labels for 64-well.

  • Drains 14–16 hidden for 52-well plates.

🛠 Enhancements in v2.4.3

• Prevented unnecessary motion on startup for CE certification.

• Default configuration values now auto-populate in new databases.

• Increased max X-controller position from 35,000 → 40,000.

• Fixed 384-well split dispense logic.

🧪 Enhancements in v2.4.2

• Mixing Amidites: Enabled creation and synthesis handling of special mixed amidites.

• Dispensing Logic: Equal volume distribution with rounding for mixed amidites.

⚙️ Enhancements in v2.4.1

• Allowed separate sequence loading for left/right plates in dual setups.

• Improved drain group logic for readiness during Map-based dispenses.

• Protocol logic updates:

  • Pulse, Hold, and Drain steps can now be set to 0.

  • Zero drain values now treated as pulses.

• Logging and encoding improvements:

  • Added version ID to logs.

  • Converted logs to UTF-8 for symbol compatibility.

• Drain group readiness now logged during dispensing.

• Added pause after dispense cycles to reduce thread race conditions.

• Bug fixes: negative dispense time error, 4-digit volume display, leak abort loop.

• UI and naming flexibility:

  • Support for custom amidite names (e.g., ABG[12]JXY[TEST]P1).

  • Reagent-only dropdowns in Backbone Mod and Protocol editors.

  • Zero-volume dispenses now skipped completely.

• Priming logic changed from time-based to volume-based (default 100µL).

• Lid sensor handling:

  • Added Prox status checks.

  • Movement restricted when lid is open.

  • Dispenses allowed regardless of tip location if lid is open.

📦 Enhancements in v2.3.4

• Draggable reagent/amidite order customization.

• Volume estimation and synthesis logs now reflect user-defined order.

• Revamped Sequence Editor UI with multi-sequence view and batch editing.

🔬 Enhancements in v2.3.3

• Added customizable Backbone Modification symbols.

• Supported new symbol handling in Sequence Editor and Run Synthesis.

🧯 Enhancements in v2.3.2

• Fixed 52-well plate dispense pattern.

🔧 Enhancements in v2.3.1

• Made swap symbol placement more flexible around amidite brackets.

• Fixed incorrect labeling on Priming screen (tip #58 showed tip #59’s reagent).

🧪 Enhancements in v2.3.0

• Introduced 52-well plate support across Configuration, Manual Control, and Run Synthesis.

• Enabled 4-digit dispense volumes (up to 9999µL).